Effects of benzyl alcohol on enzyme activities and D-glucose transport in kidney brush-border membranes

Biochim Biophys Acta. 1986 May 28;857(2):131-8. doi: 10.1016/0005-2736(86)90340-8.

Abstract

Addition of increasing amounts of benzyl alcohol progressively reduced the steady-state anisotropies of diphenylhexatriene and trimethylammoniumdiphenylhexatriene in brush-border membranes from rat kidney. The decrease in order of membrane lipids, equivalent for 50 mM benzyl alcohol to that produced by a rise in temperature of approx. 6 degrees C, had no effect on the activities of alkaline phosphatase or gamma-glutamyltranspeptidase. On the other hand, benzyl alcohol markedly inhibited the D-glucose uptakes measured in the presence of a 100 mM sodium gradient. For concentrations less than 30 mM, benzyl alcohol reduced the Jmax without significant effects on Km, 22Na+ uptake or the vesicular volume of brush-border preparations. Comparable results were obtained substituting octanol for benzyl alcohol. Our data strongly suggest that, at constant temperature, the D-glucose carrier present in renal brush-border membranes is extremely sensitive to variations in membrane physical state.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Octanol
  • Alkaline Phosphatase / metabolism
  • Animals
  • Benzyl Alcohol
  • Benzyl Alcohols / pharmacology*
  • Benzyl Compounds / pharmacology*
  • Diphenylhexatriene / analogs & derivatives
  • Dose-Response Relationship, Drug
  • Glucose / metabolism
  • Kidney / drug effects
  • Kidney / enzymology
  • Kidney / ultrastructure*
  • Kinetics
  • Male
  • Mathematics
  • Membranes / drug effects
  • Membranes / enzymology
  • Microvilli / drug effects
  • Microvilli / enzymology
  • Monosaccharide Transport Proteins / metabolism*
  • Octanols / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Sodium / metabolism
  • gamma-Glutamyltransferase / metabolism

Substances

  • Benzyl Alcohols
  • Benzyl Compounds
  • Monosaccharide Transport Proteins
  • Octanols
  • Diphenylhexatriene
  • 1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene
  • Sodium
  • gamma-Glutamyltransferase
  • Alkaline Phosphatase
  • Glucose
  • Benzyl Alcohol
  • 1-Octanol