Green synthesis of silver nanoparticles using Prosopis juliflora bark extract: reaction optimization, antimicrobial and catalytic activities

Artif Cells Nanomed Biotechnol. 2018 Aug;46(5):985-993. doi: 10.1080/21691401.2017.1354302. Epub 2017 Jul 18.


In the present study, silver nanoparticles (PJB-AgNPs) have been biosynthesized employing Prosopis juliflora bark extract. The biosynthesis of silver nanoparticles was monitored on UV-vis spectrophotometer. The size, charge and polydispersity index (PDI) of PJB-AgNPs were determined using dynamic light scattering (DLS). Different parameters dictating the size of PJB-AgNPs were explored. Nanoparticles biosynthesis optimization studies suggested efficient synthesis of highly dispersed PJB-AgNPs at 25 °C when 9.5 ml of 1 mM AgNO3 was reduced with 0.5 ml of bark extract for 40 min. Characterization of PJB-AgNPs by SEM showed spherical-shaped nanoparticles with a size range ∼10-50 nm along with a hydrodynamic diameter of ∼55 nm as evaluated by DLS. Further, characterizations were done by FTIR and EDS to evaluate the functional groups and purity of PJB-AgNPs. The antibacterial potential of PJB-AgNPs was tested against E. coli and P. aeruginosa. The PJB-AgNPs remarkably exhibited anticancer activity against A549 cell line as evidenced by Alamar blue assay. The dye degradation activity was also evaluated against 4-nitrophenol that has carcinogenic effect. The results thus obtained suggest application of PJB-AgNPs as antimicrobial, anticancer and catalytic agents.

Keywords: Prosopis juliflora; anticancer; antimicrobial; dye degradation; silver nanoparticles.

MeSH terms

  • A549 Cells
  • Anti-Bacterial Agents / chemical synthesis
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology
  • Catalysis
  • Escherichia coli / drug effects
  • Green Chemistry Technology
  • Humans
  • Metal Nanoparticles / chemistry*
  • Microbial Sensitivity Tests
  • Microwaves
  • Nanotechnology*
  • Plant Bark / chemistry*
  • Plant Extracts / chemistry*
  • Prosopis / chemistry*
  • Pseudomonas aeruginosa / drug effects
  • Silver / chemistry*
  • Silver / pharmacology*


  • Anti-Bacterial Agents
  • Antineoplastic Agents
  • Plant Extracts
  • Silver