Reciprocal Influence of hMSCs/HaCaT Cultivated on Electrospun Scaffolds

J Mater Sci Mater Med. 2017 Aug;28(8):128. doi: 10.1007/s10856-017-5941-4. Epub 2017 Jul 18.

Abstract

Here, we investigated the synergistic effect of electrospun nanofibrous scaffolds made of gelatin /sulfated hyaluronan (sHA) or native hyaluronan (HA)/chondroitin sulfate (CS) and, keratinocytes (HaCaT)-human mesenchymal stem cells (hMSCs) contact co-culture on epithelial differentiation of hMSCs. The hMSCs were co-cultured in contact with HaCaT cells for 5 days on electrospun scaffold. Results show that electrospun scaffolds containing sulfated glycosaminoglycans (GAGs) stimulate epithelial differentiation in terms of various protein expression markers (keratin 14, ΔNp63α and Pan-cytokeratin) and gene expression of several dermal proteins (keratin 14, ΔNp63α). Electrospun scaffold independent of GAGs alone did not affect the epithelial differentiation of hMSCs but combination of keratinocyte-hMSC contact co-culture and electrospun scaffold promotes the epithelial differentiation of hMSCs.

MeSH terms

  • Cell Communication / physiology*
  • Cell Differentiation
  • Cells, Cultured
  • Coculture Techniques / instrumentation
  • Coculture Techniques / methods
  • Electroplating / methods
  • Gene Expression Regulation
  • Humans
  • Keratinocytes / cytology*
  • Keratinocytes / physiology*
  • Materials Testing
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / physiology*
  • Nanofibers / chemistry
  • Tissue Scaffolds* / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Tumor Suppressor Proteins / genetics
  • Tumor Suppressor Proteins / metabolism

Substances

  • TP63 protein, human
  • Transcription Factors
  • Tumor Suppressor Proteins