Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data

Sci Rep. 2017 Jul 19;7(1):5913. doi: 10.1038/s41598-017-06174-3.


Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spike-in oligonucleotides (sRNA spike-ins) that enable absolute normalization of sRNA-Seq data across independent experiments, as well as the genome-wide estimation of sRNA:mRNA stoichiometries when used together with mRNA spike-in oligonucleotides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Oligonucleotides / metabolism*
  • RNA / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Analysis, RNA*


  • Oligonucleotides
  • RNA, Messenger
  • RNA