CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production

Sci Rep. 2017 Jul 19;7(1):5922. doi: 10.1038/s41598-017-06397-4.


Eosinophils and their associated cytokines IL-4 and IL-5 are emerging as central orchestrators of the immune-metabolic axis. Herein, we demonstrate that cross-talk between the Ig-superfamily receptor CD300f and IL-5 is a key checkpoint that modifies the ability of eosinophils to regulate metabolic outcomes. Generation of Il5 Tg /Cd300f -/- mice revealed marked and distinct increases in eosinophil levels and their production of IL-4 in the white and brown adipose tissues. Consequently, Il5 Tg /Cd300f -/- mice had increased alternatively activated macrophage accumulation in the adipose tissue. Cd300f -/- mice displayed age-related accumulation of eosinophils and macrophages in the adipose tissue and decreased adipose tissue weight, which was associated with decreased diet-induced weight gain and insulin resistance. Notably, Il5 Tg /CD300f -/- were protected from diet-induced weight gain and glucose intolerance. These findings highlight the cross-talk between IL-5 receptor and CD300f as a novel pathway regulating adipose tissue eosinophils and offer new entry points for therapeutic intervention for obesity and its complications.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology*
  • Animals
  • Endothelial Cells / metabolism
  • Eosinophils / metabolism*
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Glucose Intolerance / metabolism
  • Glucose Intolerance / pathology
  • Interleukin-4 / metabolism*
  • Interleukin-5 / metabolism*
  • Ligands
  • Macrophages / metabolism
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt / metabolism
  • Receptors, Immunologic / metabolism*
  • Receptors, Interleukin-5 / metabolism
  • Weight Gain


  • CLM-1 protein, mouse
  • Interleukin-5
  • Ligands
  • Receptors, Immunologic
  • Receptors, Interleukin-5
  • Interleukin-4
  • Proto-Oncogene Proteins c-akt
  • Extracellular Signal-Regulated MAP Kinases