Immunomodulatory effects of hydroxyethylated Hericium erinaceus polysaccharide on macrophages RAW264.7

Int J Biol Macromol. 2017 Dec;105(Pt 1):879-885. doi: 10.1016/j.ijbiomac.2017.07.104. Epub 2017 Jul 18.


Hericium erinaceus polysaccharide (HEP) has been shown to possess a variety of biological activities. In present study, HEP was successfully modified to obtain its hydroxyethylated derivative hHEP. Its potential immunomodulatory activities on RAW264.7 macrophages were investigated. Results showed that the hHEP were significantly stronger than that of the corresponding unmodified polysaccharide, HEP. Meanwhile, the NO, IL-6 and TNF-α production activities of macrophages were enhanced in the RAW264.7 macrophages by stimulation of hHEP. In addition, the hHEP increase significantly higher iNOS expression than HEP. These results indicated that the hydroxyethylated derivative hHEP could enhance the activation of peritoneal macrophages, and hydroxyethylation modification can enhance the immunomodulation function of HEP.

Keywords: Hericium erinaceus polysaccharide; Hydroxyethylation; Immunomodulation.

MeSH terms

  • Animals
  • Basidiomycota / chemistry*
  • Cell Survival / drug effects
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / pharmacology*
  • Immunologic Factors / chemistry*
  • Immunologic Factors / pharmacology*
  • Interleukin-6 / metabolism
  • Macrophages / cytology
  • Macrophages / drug effects*
  • Macrophages / immunology*
  • Macrophages / metabolism
  • Mice
  • Nitric Oxide / metabolism
  • Phagocytosis / drug effects
  • RAW 264.7 Cells
  • Tumor Necrosis Factor-alpha / metabolism


  • Fungal Polysaccharides
  • Immunologic Factors
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide