Comment on "The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport"

Science. 2017 Jul 21;357(6348):eaan2396. doi: 10.1126/science.aan2396.


O'Brien et al (Research Article, 24 February 2017, eaag1789) proposed a novel mechanism of primase function based on redox activity of the iron-sulfur cluster buried inside the C-terminal domain of the large primase subunit (p58C). Serious problems in the experimental design and data interpretation raise concerns about the validity of the conclusions.

Publication types

  • Research Support, N.I.H., Extramural
  • Comment

MeSH terms

  • Biological Transport
  • DNA
  • DNA Primase / genetics*
  • Humans
  • Iron-Sulfur Proteins / genetics
  • Oxidation-Reduction*


  • Iron-Sulfur Proteins
  • DNA
  • DNA Primase