Idiomarina andamanensis sp. nov., an alkalitolerant bacterium isolated from Andaman Sea water

Antonie Van Leeuwenhoek. 2017 Dec;110(12):1581-1592. doi: 10.1007/s10482-017-0908-5. Epub 2017 Jul 20.

Abstract

Two closely related aerobic, Gram-negative rod shaped bacteria (strain W5T and W3) were isolated from Andaman Sea. Heterotrophic growth on marine agar was observed at 15-45 °C and pH 6-10. Strain W5T showed maximum 16S rRNA sequence similarity of 99.58% with Idiomarina marina JCM 15083T. DNA fingerprinting analysis by ERIC-REP PCR, PFGE and MLSA revealed differences in banding patterns, also DNA-DNA hybridization values were well below 70% confirming W5T to be a new species. DNA G+C content was 46.7 mol%. Major fatty acids were iso-C15:0, iso-C17:0, iso-C17:1 ω9c, iso-C13:0 3OH, iso-C11:0 3OH and C16:0. Polar lipids included phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) diphosphatidylglycerol (DPG) phospholipid (PL) two aminolipids (AL) and two unidentified lipids (L1-2). Q-8 is the predominant ubiquinone. On the basis of polyphasic taxonomic study, strain W5T is considered to be representative of a new species of the genus Idiomarina, for which the name Idiomarina andamanensis sp. nov. is being proposed. The type strain W5T (= LMG 29773T = JCM 31645T) was isolated from Andaman Sea.

Keywords: 16S rRNA sequence; Andaman Sea; DNA–DNA hybridization; ERIC-PCR; Idiomarina andamanensis sp. nov; PFGE; REP-PCR.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Gammaproteobacteria / chemistry
  • Gammaproteobacteria / classification*
  • Gammaproteobacteria / genetics*
  • Gammaproteobacteria / isolation & purification
  • Metabolomics / methods
  • Multilocus Sequence Typing
  • Nucleic Acid Hybridization
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Water Microbiology*

Substances

  • RNA, Ribosomal, 16S