Intracellular Reassociation of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells

Methods Mol Biol. 2017;1632:269-283. doi: 10.1007/978-1-4939-7138-1_18.


Human immunodeficiency virus Type 1 (HIV-1) is the major cause of acquired immune deficiency syndrome (AIDS). In 2014, it was estimated that 1.2 million people died from AIDS-related illnesses. RNA interference-based therapy to block HIV replication is a field that, as of now, is without any FDA-approved drugs available for clinical use. In this chapter we describe a protocol for testing and utilizing a new approach that relies on reassociation of RNA-DNA hybrids activating RNAi and blocking HIV replication in human cells.

Keywords: Dicer substrate RNA; HIV-1; Hybrids reassociation; RNA interference; RNA nanotechnology; RNA–DNA hybrids.

MeSH terms

  • Cell Line
  • DNA* / chemistry
  • HIV-1 / physiology*
  • Humans
  • Nanotechnology
  • RNA Interference*
  • RNA* / chemistry
  • Transfection
  • Virus Replication / genetics


  • RNA
  • DNA