Preparation of a Conditional RNA Switch

Methods Mol Biol. 2017;1632:303-324. doi: 10.1007/978-1-4939-7138-1_20.

Abstract

RNA has gained great interest for use in biomedical and therapeutic applications. This is due in part to RNA's ability to perform multiple functions, including the regulation of endogenously expressed genes. However, the ability of RNA based drugs to distinguish target diseased cells from healthy tissue remains challenging. Here we present methods for the production of a recently developed conditional RNA switch that releases a Dicer substrate RNA in response to interaction with a specific RNA biomarker.

Keywords: Conditional RNA; Nanomedicine; Nanotechnology; RNA; RNA switch; RNase H; Toehold.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Cell Line, Tumor
  • Electrophoresis, Polyacrylamide Gel
  • Gene Knockdown Techniques
  • Gene Silencing
  • Humans
  • Nanomedicine
  • Nanotechnology
  • Nucleic Acid Conformation
  • RNA / chemistry
  • RNA / genetics*
  • Ribonuclease H / chemistry
  • Ribonuclease H / metabolism
  • Riboswitch*

Substances

  • Riboswitch
  • RNA
  • Ribonuclease H