Transformation of Schizosaccharomyces pombe in a 96-Well Format

Cold Spring Harb Protoc. 2018 Jan 2;2018(1). doi: 10.1101/pdb.prot091942.


This protocol describes chemical transformation of Schizosaccharomyces pombe with linear DNA in a 96-well format. This procedure has been successfully used for large-scale strain construction in fission yeast.

MeSH terms

  • Genetics, Microbial / methods*
  • High-Throughput Screening Assays / methods
  • Molecular Biology / methods*
  • Schizosaccharomyces / genetics*
  • Transformation, Genetic*