DNA Preparation from Schizosaccharomyces pombe

Cold Spring Harb Protoc. 2018 Jan 2;2018(1). doi: 10.1101/pdb.prot091959.


This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.

MeSH terms

  • DNA, Fungal / isolation & purification*
  • Genetics, Microbial / methods*
  • Genotyping Techniques
  • Molecular Biology / methods*
  • Mycological Typing Techniques
  • Polymerase Chain Reaction
  • Schizosaccharomyces / genetics*


  • DNA, Fungal