Dietary administration of tetrodotoxin and its putative biosynthetic intermediates to the captive-reared non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster

Yuta Kudo; Chikafumi Chiba; Keiichi Konoki; Yuko Cho; Mari Yotsu-Yamashita

doi:10.1016/j.toxicon.2017.07.016

Dietary administration of tetrodotoxin and its putative biosynthetic intermediates to the captive-reared non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster

Toxicon. 2017 Oct:137:78-82. doi: 10.1016/j.toxicon.2017.07.016. Epub 2017 Jul 20.

Authors

Yuta Kudo¹, Chikafumi Chiba², Keiichi Konoki¹, Yuko Cho¹, Mari Yotsu-Yamashita³

Affiliations

¹ Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aza-Aoba, Aoba-ku, Sendai, Miyagi, 980-0845, Japan.
² Faculty of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.
³ Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aza-Aoba, Aoba-ku, Sendai, Miyagi, 980-0845, Japan. Electronic address: mari.yamashita.c1@tohoku.ac.jp.

PMID: 28734983
DOI: 10.1016/j.toxicon.2017.07.016

Abstract

The origin of tetrodotoxin (TTX) in amphibians has long been disputed. In this study, TTX and its putative biosynthetic intermediates or shunt compounds (4,9-anhydro-10-hemiketal-5-deoxyTTX and Cep-242) were dietary administered to the captive-reared, non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster. After 4 weeks, the ingested compounds were detected mainly in the newt body using liquid chromatography-mass spectrometry (LC-MS), while these compounds were not converted into other TTX analogues in newts.

Keywords: Cynops pyrrhogaster; LC-MS; Newt; Tetrodotoxin; Toxin administration.

MeSH terms

Animals
Chromatography, Liquid
Mass Spectrometry
Salamandridae / metabolism*
Tetrodotoxin / administration & dosage*
Tetrodotoxin / analogs & derivatives*

Substances

Tetrodotoxin