A nanosecond time-resolved XFEL analysis of structural changes associated with CO release from cytochrome c oxidase

Sci Adv. 2017 Jul 14;3(7):e1603042. doi: 10.1126/sciadv.1603042. eCollection 2017 Jul.

Abstract

Bovine cytochrome c oxidase (CcO), a 420-kDa membrane protein, pumps protons using electrostatic repulsion between protons transferred through a water channel and net positive charges created by oxidation of heme a (Fe a ) for reduction of O2 at heme a3 (Fe a3). For this process to function properly, timing is essential: The channel must be closed after collection of the protons to be pumped and before Fe a oxidation. If the channel were to remain open, spontaneous backflow of the collected protons would occur. For elucidation of the channel closure mechanism, the opening of the channel, which occurs upon release of CO from CcO, is investigated by newly developed time-resolved x-ray free-electron laser and infrared techniques with nanosecond time resolution. The opening process indicates that CuB senses completion of proton collection and binds O2 before binding to Fe a3 to close the water channel using a conformational relay system, which includes CuB, heme a3, and a transmembrane helix, to block backflow of the collected protons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Monoxide / chemistry*
  • Carbon Monoxide / metabolism*
  • Electron Transport Complex IV / chemistry*
  • Electron Transport Complex IV / metabolism*
  • Heme / chemistry
  • Heme / metabolism
  • Hydrogen Bonding
  • Models, Molecular
  • Molecular Conformation
  • Molecular Structure
  • Oxidation-Reduction
  • Photolysis
  • Structure-Activity Relationship

Substances

  • Heme
  • Carbon Monoxide
  • Electron Transport Complex IV