A unique polysaccharide purified from Hericium erinaceus mycelium prevents oxidative stress induced by H2O2 in human gastric mucosa epithelium cell

PLoS One. 2017 Jul 24;12(7):e0181546. doi: 10.1371/journal.pone.0181546. eCollection 2017.

Abstract

Hericium erinaceus (HE) has been used both as a traditional Chinese medicine and home remedy for treatment of gastric and duodenal ulcers and gastritis. EP-1, a purified polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for HE anti-gastritis activity. Because oxidative stress has been implicated as a pathogenic cause of gastritis and gastric ulcers, EP-1 antioxidant properties were systematically examined in vitro using the human gastric mucosal epithelial cell line, GES-1. Results showed that EP-1 possessed higher oxygen radical absorbance capacity (ORAC) and 2-3 times higher ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals than a hot water extract of commercially available HE fruiting body. A crude mycelial polysaccharide (CMPS) extract of HE, from which EP-1 was purified, showed slightly stronger radical scavenging activity and ORAC than EP-1, with the exception of DPPH-scavenging activity. Antioxidant activities of these extracts were further studied using hydrogen peroxide (H2O2)-abused GES-1 cells; EP-1 dose-dependently preserved cell viability of abused cells as assessed via MTT assay. Moreover, FACS analysis revealed that EP-1 prevented H2O2-induced apoptotic cell death by inhibiting activation of apoptotic cellular signals within mitochondria-dependent apoptotic pathways. CMPS also prevented H2O2-induced oxidative stress, but to a lesser degree than did EP-1, even though CMPS exhibited comparable or stronger in vitro antioxidant activity than did EP-1.

MeSH terms

  • Animals
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Apoptosis / drug effects
  • Basidiomycota / chemistry*
  • Cell Line
  • Female
  • Gastric Mucosa / drug effects*
  • Gastric Mucosa / metabolism
  • Gastric Mucosa / pathology
  • Gastritis / metabolism
  • Gastritis / pathology
  • Gastritis / prevention & control*
  • Humans
  • Hydrogen Peroxide / metabolism
  • Male
  • Mice
  • Mycelium / chemistry
  • Oxidative Stress / drug effects*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology
  • Polysaccharides / therapeutic use*

Substances

  • Antioxidants
  • Polysaccharides
  • Hydrogen Peroxide

Grant support

This work was financial supported by research grants from the National Natural Science Foundation of China(NSFC 81603276) and supported by the Foundation of Health and Family Planning Commission of Jilin Province (Grant No. 20152F2C01)s work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.