Structural basis for dolichylphosphate mannose biosynthesis

Nat Commun. 2017 Jul 25;8(1):120. doi: 10.1038/s41467-017-00187-2.


Protein glycosylation is a critical protein modification. In biogenic membranes of eukaryotes and archaea, these reactions require activated mannose in the form of the lipid conjugate dolichylphosphate mannose (Dol-P-Man). The membrane protein dolichylphosphate mannose synthase (DPMS) catalyzes the reaction whereby mannose is transferred from GDP-mannose to the dolichol carrier Dol-P, to yield Dol-P-Man. Failure to produce or utilize Dol-P-Man compromises organism viability, and in humans, several mutations in the human dpm1 gene lead to congenital disorders of glycosylation (CDG). Here, we report three high-resolution crystal structures of archaeal DPMS from Pyrococcus furiosus, in complex with nucleotide, donor, and glycolipid product. The structures offer snapshots along the catalytic cycle, and reveal how lipid binding couples to movements of interface helices, metal binding, and acceptor loop dynamics to control critical events leading to Dol-P-Man synthesis. The structures also rationalize the loss of dolichylphosphate mannose synthase function in dpm1-associated CDG.The generation of glycolipid dolichylphosphate mannose (Dol-P-Man) is a critical step for protein glycosylation and GPI anchor synthesis. Here the authors report the structure of dolichylphosphate mannose synthase in complex with bound nucleotide and donor to provide insight into the mechanism of Dol-P-Man synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Archaeal Proteins / chemistry
  • Archaeal Proteins / genetics
  • Archaeal Proteins / metabolism*
  • Binding Sites / genetics
  • Biocatalysis
  • Crystallography, X-Ray
  • Mannose / biosynthesis*
  • Mannose / chemistry
  • Mannosyltransferases / chemistry
  • Mannosyltransferases / genetics
  • Mannosyltransferases / metabolism*
  • Models, Molecular
  • Protein Domains
  • Pyrococcus furiosus / enzymology
  • Pyrococcus furiosus / genetics
  • Pyrococcus furiosus / metabolism*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid


  • Archaeal Proteins
  • Recombinant Proteins
  • Mannosyltransferases
  • protein O-mannosyltransferase
  • Mannose