We studied the effect of GlcNAc-, Man-, and Gal-specific lectins isolated from cell cytosol, nuclear membrane, and nuclear matrix of calf brain cortex, lyophilized, and stored for 5 years on proliferation activity of human peripheral blood lymphocytes and on hemagglutination activity of trypsinized rabbit erythrocytes. Human peripheral blood lymphocytes treated lyophilized lectins demonstrated lower proliferation activity than lymphocytes treated with concanavalin A (positive control), but higher than control lymphocytes (incubated with saline). Lectins produced no effect on hemagglutination activity.
Keywords: MTT assay; calf brain lectins; hemagglutination activity; proliferation of human peripheral blood lymphocytes.