Genomic characterization reconfirms the taxonomic status of Lactobacillus parakefiri

Biosci Microbiota Food Health. 2017;36(3):129-134. doi: 10.12938/bmfh.16-026. Epub 2017 Mar 4.


Whole-genome sequencing was performed for Lactobacillus parakefiri JCM 8573T to confirm its hitherto controversial taxonomic position. Here, we report its first reliable reference genome. Genome-wide metrics, such as average nucleotide identity and digital DNA-DNA hybridization, and phylogenomic analysis based on multiple genes supported its taxonomic status as a distinct species in the genus Lactobacillus. The availability of a reliable genome sequence will aid future investigations on the industrial applications of L. parakefiri in functional foods such as kefir grains.

Keywords: Lactobacillus parakefiri; lactic acid bacteria; taxonomy; whole-genome sequence.