MoS 2-LA-PEI nanocomposite carrier for real-time imaging of ATP metabolism in glioma stem cells co-cultured with endothelial cells on a microfluidic system

Biosens Bioelectron. 2018 Jan 15;99:142-149. doi: 10.1016/j.bios.2017.07.046. Epub 2017 Jul 19.

Abstract

Stimuli-responsive carriers have extensively attracted attention in recent years. However, long-term and real-time tracking ability with stimuli-responsive carrier is still in its infant stage due to the limitations such as, low efficacy, instability and cytotoxicity in a bio-environment. In this work, we developed a reduction-sensitive carrier composed of lipoic acid-modified low molecular weight polyethyleneimine (LA-PEI) and large surface ratio MoS2 nanosheet integrated via disulfide bond to mimic a high molecular weight PEI. The positively charged carriers loading negatively charged aptamer enter the cells for a real time long-term tracking of adenosine triphosphate (ATP) metabolism in glioma stem cells (GSCs) when stimulated by TGFβ factor secreted from HUVECs. We envision that MoS2-LA-PEI carrier has a promising potential for delivery and monitoring the changes in live cells with low cytotoxicity and high efficiency.

Keywords: ATP metabolism; Low cytotoxicity; PEI nanocomposite; Stimuli-responsive carrier.

MeSH terms

  • Adenosine Triphosphate / isolation & purification
  • Adenosine Triphosphate / metabolism*
  • Biosensing Techniques*
  • Coculture Techniques
  • Endothelial Cells / metabolism*
  • Endothelial Cells / pathology
  • Glioma / metabolism*
  • Glioma / pathology
  • HeLa Cells
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Lab-On-A-Chip Devices
  • Nanocomposites / chemistry
  • Neoplastic Stem Cells / metabolism
  • Neoplastic Stem Cells / pathology
  • Polyethyleneimine / chemistry
  • Transforming Growth Factor beta1 / chemistry
  • Transforming Growth Factor beta1 / genetics

Substances

  • Transforming Growth Factor beta1
  • Adenosine Triphosphate
  • Polyethyleneimine