Development and validation of a new loop-mediated isothermal amplification for detection of pathogenic Leptospira species in clinical materials

J Microbiol Methods. 2017 Oct:141:55-59. doi: 10.1016/j.mimet.2017.07.010. Epub 2017 Jul 26.

Abstract

Leptospirosis, a global zoonotic disease, is often neglected but has a significant impact on human health. Here we reported a new loop mediated isothermal amplification (LAMP) assay targeting lipL32 gene of pathogenic Leptospira spp. Polymerase chain reaction (PCR), nested-PCR and real-time PCR assays were included in this study for the comparison of analytic and diagnostic sensitivity and specificity. LipL32 LAMP we designed enables detection of 10 copies of L. interrogans and has a higher diagnostic sensitivity (91.67%) and specificity (100%) than other PCR-based methods. The high sensitivity, specificity and flexible reaction conditions of the lipL32 LAMP assay makes it feasible for resource-limited countries and on-site application.

Keywords: DNA detection; Leptospira; Loop-mediated isothermal amplification (LAMP).

Publication types

  • Validation Study

MeSH terms

  • Animals
  • Cat Diseases / diagnosis*
  • Cat Diseases / microbiology
  • Cats
  • DNA, Bacterial / genetics
  • Leptospira / genetics*
  • Leptospira / pathogenicity
  • Leptospirosis / diagnosis
  • Leptospirosis / microbiology
  • Leptospirosis / urine
  • Leptospirosis / veterinary*
  • Nucleic Acid Amplification Techniques / methods*
  • Real-Time Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Temperature

Substances

  • DNA, Bacterial