A novel method of gene transduction to the murine endometrium using in vivo electroporation

J Vet Med Sci. 2017 Sep 29;79(9):1573-1577. doi: 10.1292/jvms.17-0220. Epub 2017 Jul 31.

Abstract

To investigate the molecular pathways involved in successful embryo implantation in mammals, we developed a novel method for gene transduction into the murine endometrium using in vivo electroporation. Plasmid DNA with an enhanced green fluorescence protein (EGFP) gene was injected into the uterine cavity of non-pregnant female mice, and electrical pulses were subsequently applied to the uterine horn using plate electrodes. EGFP expression was found only in the uterine luminal epithelium (LE), but not in the stroma. EGFP fluorescence in the LE was limited to the site where the positive side of the electrodes was placed during electric stimulation. These results demonstrated that our novel method enabled us to transduce a gene into a desired location of the murine uterus.

Keywords: EGFP; embryo implantation; in vivo electroporation; uterus.

MeSH terms

  • Animals
  • Electroporation / methods*
  • Endometrium / metabolism*
  • Female
  • Gene Expression Regulation
  • Gene Transfer Techniques
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Mice
  • Plasmids
  • Pregnancy

Substances

  • enhanced green fluorescent protein
  • Green Fluorescent Proteins