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. 2017 Jul 18;10:267-273.
doi: 10.2147/CCID.S141845. eCollection 2017.

Oral Hyaluronan Relieves Wrinkles: A Double-Blinded, Placebo-Controlled Study Over a 12-week Period

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Free PMC article

Oral Hyaluronan Relieves Wrinkles: A Double-Blinded, Placebo-Controlled Study Over a 12-week Period

Mariko Oe et al. Clin Cosmet Investig Dermatol. .
Free PMC article

Abstract

Background: Hyaluronan (HA) has critical moisturizing property and high water retention capacity especially for human skin. This study aimed to evaluate the effect of oral intake of HA.

Methods: The mean molecular weight (MW) of HA is 2 k and 300 k. Sixty Japanese male and female subjects aged 22-59 years who presented with crow's feet wrinkles were randomly assigned to the HA 2 k or HA 300 k at 120 mg/day or the placebo group. The subjects were administered HA at a rate of 120 mg/day or a placebo for 12 weeks. The skin wrinkles were evaluated by image analysis of skin wrinkle replicas, and their skin condition was evaluated using a questionnaire survey.

Results: During the study period, the HA groups showed better level of the whole sulcus volume ratio, wrinkle area ratio, and wrinkle volume ratio than the placebo group. After 8 weeks of ingestion, the HA 300 k group showed significantly diminished wrinkles compared with the placebo group. Skin luster and suppleness significantly improved after 12 weeks in all groups compared with the baseline.

Conclusion: The results suggest that oral HA (both HA 2 k and HA 300 k) inhibits skin wrinkles and improves skin condition.

Keywords: dietary supplement; hyaluronic acid; molecular weight; skin; wrinkle volume.

Conflict of interest statement

Disclosure Ms. Mariko Oe, Mr. Seigo Sakai, Mr. Hideto Yoshida, Ms. Haruna Kaneda, Ms. Nao Okado, Dr. Yasunobu Masuda are employees of Kewpie Coroporation. MD Osamu Urushibata is a professor of Toho University. The authors report no other conflicts of interest in this work.

Figures

Figure 1
Figure 1
Changes from baseline in the analysis results of the wrinkle replica with time after ingestion of HA or the placebo. Notes: Results of (A) ⊿whole sulcus volume ratio, (B) ⊿wrinkle area ratio, and (C) ⊿wrinkle volume ratio relative to the baseline are shown. ○ indicates the placebo group; ■ indicates the HA 2 k group; □ indicates the HA 300 k group. Tukey’s test was used to compare the three groups. Data are presented as mean ± SE. *p < 0.05, **p < 0.1 vs placebo group. Abbreviations: HA, hyaluronan; SE, standard error; AU, arbitrary unit.
Figure 2
Figure 2
Changes in the subjective skin condition with time after ingestion of HA or the placebo. Notes: Results of (A) ⊿skin luster score, (B) ⊿skin suppleness score, and (C) ⊿wrinkles score relative to the baseline are shown. ○ indicates the placebo group; ■ indicates the HA 2 k group; □ indicates the HA 300 k group. The Wilcoxon signed-rank test was used to compare the three groups. Data are presented as mean ± SE. Abbreviations: HA, hyaluronan; SE, standard error.
Figure 3
Figure 3
Typical microscope images of each group. Notes: Microscopic images of the outer corner of the eye prior to ingestion and after 8 weeks of ingestion are shown for each group. Abbreviation: HA, hyaluronan.

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