In Vitro Mechanical Property Evaluation of Chitosan-Based Hydrogels Intended for Vascular Graft Development

J Cardiovasc Transl Res. 2017 Dec;10(5-6):480-488. doi: 10.1007/s12265-017-9763-z. Epub 2017 Jul 31.


Vascular grafts made of synthetic polymers perform poorly in cardiac and peripheral bypass applications. In these applications, chitosan-based materials can be produced and shaped to provide a novel scaffold for vascular tissue engineering. The goal of this study was to evaluate in vitro the mechanical properties of a novel chitosan formulation to assess its potential for this scaffold. Two chitosan-based hydrogel tubes were produced by modulating chitosan concentration. Based on the standard ISO 7198:1998, the hydrogel tubes were characterized in vitro in terms of suture retention strength, tensile strength, compliance, and burst pressure. By increasing chitosan concentration, suture retention value increased to reach 1.1 N; average burst strength and elastic moduli also increased significantly. The compliance seemed to exhibit a low value for chitosan tubes of high concentration. By modulating chitosan concentration, we produced scaffolds with suitable mechanical properties to be implanted in vivo and withstand physiological blood pressures.

Keywords: Chitosan; Hydrogels; In vitro; Mechanical properties; Vascular graft development.

MeSH terms

  • Blood Vessel Prosthesis Implantation / instrumentation*
  • Blood Vessel Prosthesis*
  • Chitosan / chemistry*
  • Elastic Modulus
  • Hydrogels
  • Materials Testing
  • Pressure
  • Prosthesis Design
  • Prosthesis Failure
  • Stress, Mechanical
  • Suture Techniques
  • Tensile Strength
  • Tissue Engineering / methods*
  • Tissue Scaffolds*


  • Hydrogels
  • Chitosan