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. 2017 Oct;11(2):211-214.
doi: 10.1007/s12104-017-9750-y. Epub 2017 Aug 1.

Backbone and Side Chain Resonance Assignments for a Structured Domain Within Atg32

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Free PMC article

Backbone and Side Chain Resonance Assignments for a Structured Domain Within Atg32

Xue Xia et al. Biomol NMR Assign. .
Free PMC article

Abstract

Autophagy is a catabolic cellular process that targets cytosolic material, including mitochondria, to the vacuole or lysosomes for degradation. The selective degradation of mitochondria by autophagy is termed mitophagy. Dysfunctional mitophagy, which leads to the accumulation of damaged mitochondria, has been implicated in Parkinson's disease, cancer, cardiac disease and metabolic disease. In Saccharomyces cerevisiae, mitophagy is initiated by the autophagy receptor Atg32, an outer mitochondrial membrane protein. A lack of structural information for Atg32 has hindered our understanding of the molecular mechanisms of mitophagy initiation. To gain new structural insight into Atg32, we have identified the location of a structured domain within the cytosolic region of Atg32 and completed the backbone and side chain resonance assignments for this domain.

Keywords: Atg32; Autophagy; Mitophagy; NMR.

Figures

Figure 1
Figure 1
Annotated 2D [1H 15N] HSQC spectrum recorded on a Bruker Avance 700 MHz magnet at 308 K. Residues are labeled with the single letter code and the number at which they appear in the Atg32 sequence. Atg32200-341 contains no tryptophan residues.
Figure 2
Figure 2
Secondary structure probabilities as calculated using the TALOS-N webserver. Blue bars represent the β-strand probabilities and red bars represent the α-helix probabilities.

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