Determination of fat- and water-soluble vitamins by supercritical fluid chromatography: A review

J Sep Sci. 2018 Jan;41(1):336-350. doi: 10.1002/jssc.201700598. Epub 2017 Sep 11.


Vitamins are compounds that take part in all basic functions of an organism but also are subject of number of studies performed by different researchers. Two groups of vitamins are distinguished taking into consideration their solubility. Chromatography with supercritical CO2 has found application in the determination, separation, and quantitative analyses of both fat- and water-soluble vitamins. The methods of vitamins separation have developed and improved throughout the years. Both groups of compounds were separated using supercritical fluid chromatography with different detection on different stationary phases. The main aim of this review is to provide an overview of the studies of vitamins separation that have been determined so far.

Keywords: fat-soluble vitamins; supercritical fluid chromatography; ultra-performance convergence chromatography; water-soluble vitamins.

Publication types

  • Review

MeSH terms

  • Animals
  • Carbon Dioxide / chemistry
  • Carotenoids / chemistry
  • Chromatography, Supercritical Fluid*
  • Fats / chemistry
  • Humans
  • Pressure
  • Solubility
  • Temperature
  • Viscosity
  • Vitamin A / analysis*
  • Vitamins / analysis*
  • Vitamins / chemistry
  • Water / chemistry


  • Fats
  • Vitamins
  • Water
  • Vitamin A
  • Carbon Dioxide
  • Carotenoids