Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae)

Nat Prod Res. 2018 Aug;32(16):1991-1995. doi: 10.1080/14786419.2017.1359171. Epub 2017 Aug 3.

Abstract

The antioxidant activities of crude extract fractions using Hexane, Chloroform, Ethyl Acetate, Butanol and Water of Clematis orientalis and Clematis ispahanica were investigated. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay and the ferric reducing/antioxidant potential (FRAP) were used to evaluate the antioxidant capacity. The total phenolics were found to be 4.37-9.38 and 1.32-11.37 mg gallic acid equivalents (GAE)/g in different fractions for C. orientalis and C. ispahanica, respectively. The ethyl acetate fraction of C. orientalis and chloroform fraction of C. ispahanica showed the highest DPPH and FRAP activities at a concentration of 300 μg/mL. The predominant phenolic compounds identified by HPLC in C. orientalis were Resorcinol (603.5 μg/g DW) in chloroform fraction and Ellagic acid (811.7 μg/g DW) in chloroform fraction of C. ispahanica.

Keywords: Clematis ispahanica; Clematis orientalis; HPLC; antioxidant activity.

MeSH terms

  • Antioxidants / chemistry
  • Antioxidants / isolation & purification*
  • Chromatography, High Pressure Liquid
  • Clematis / chemistry*
  • Flavonoids / chemistry*
  • Phenols / chemistry*
  • Plant Extracts / chemistry*
  • Ranunculaceae / chemistry
  • Solvents

Substances

  • Antioxidants
  • Flavonoids
  • Phenols
  • Plant Extracts
  • Solvents