An N-methyl-D-aspartate (NMDA) Receptor Antagonist Reduces Bicuculline-Induced Depolarization Shifts in Neocortical Explant Cultures

Neurosci Lett. 1986 Sep 25;70(1):101-5. doi: 10.1016/0304-3940(86)90445-3.

Abstract

We investigated the actions of a specific N-methyl-D-aspartate (NMDA) antagonist, 2-amino-5-phosphonovaleric acid (2-APV), on bicuculline-induced epileptogenesis in organotypic explant cultures from neonatal rat neocortex. Explants were maintained in roller tubes for 3-5 weeks. The late, plateau phase of the intracellularly recorded paroxysmal depolarization shift was sensitive to both intracellularly injected hyperpolarizing currents and 2-APV, suggesting that this component is generated by a voltage-dependent, regenerative process that is mediated by activation of NMDA receptors. The results support the hypothesis that NMDA receptors play an important role in the generation of epileptiform activity by localized circuits of neocortical neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Amino-5-phosphonovalerate
  • Animals
  • Animals, Newborn
  • Anticonvulsants*
  • Bicuculline / antagonists & inhibitors*
  • Cerebral Cortex / drug effects*
  • Culture Techniques
  • Epilepsy / chemically induced
  • Epilepsy / prevention & control
  • Membrane Potentials / drug effects
  • Organ Specificity
  • Rats
  • Rats, Inbred Strains
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Neurotransmitter / drug effects*
  • Valine / analogs & derivatives*
  • Valine / pharmacology

Substances

  • Anticonvulsants
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Neurotransmitter
  • 2-Amino-5-phosphonovalerate
  • Valine
  • Bicuculline