Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist

J Am Chem Soc. 2017 Aug 30;139(34):11980-11988. doi: 10.1021/jacs.7b06186. Epub 2017 Aug 22.

Abstract

The ethylene-forming enzyme (EFE) from Pseudomonas syringae pv. phaseolicola PK2 is a member of the mononuclear nonheme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily. EFE converts 2OG into ethylene plus three CO2 molecules while also catalyzing the C5 hydroxylation of l-arginine (l-Arg) driven by the oxidative decarboxylation of 2OG to form succinate and CO2. Here we report 11 X-ray crystal structures of EFE that provide insight into the mechanisms of these two reactions. Binding of 2OG in the absence of l-Arg resulted in predominantly monodentate metal coordination, distinct from the typical bidentate metal-binding species observed in other family members. Subsequent addition of l-Arg resulted in compression of the active site, a conformational change of the carboxylate side chain metal ligand to allow for hydrogen bonding with the substrate, and creation of a twisted peptide bond involving this carboxylate and the following tyrosine residue. A reconfiguration of 2OG achieves bidentate metal coordination. The dioxygen binding site is located on the metal face opposite to that facing l-Arg, thus requiring reorientation of the generated ferryl species to catalyze l-Arg hydroxylation. Notably, a phenylalanyl side chain pointing toward the metal may hinder such a ferryl flip and promote ethylene formation. Extensive site-directed mutagenesis studies supported the importance of this phenylalanine and confirmed the essential residues used for substrate binding and catalysis. The structural and functional characterization described here suggests that conversion of 2OG to ethylene, atypical among Fe(II)/2OG oxygenases, is facilitated by the binding of l-Arg which leads to an altered positioning of the carboxylate metal ligand, a resulting twisted peptide bond, and the off-line geometry for dioxygen coordination.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Arginine / metabolism
  • Binding Sites
  • Carbon Dioxide / metabolism
  • Catalytic Domain
  • Ferrous Compounds / metabolism*
  • Hydroxylation
  • Ketoglutaric Acids / metabolism*
  • Lyases / chemistry
  • Lyases / metabolism*
  • Models, Molecular
  • Protein Conformation
  • Pseudomonas syringae / chemistry
  • Pseudomonas syringae / enzymology*
  • Pseudomonas syringae / metabolism
  • Substrate Specificity

Substances

  • Ferrous Compounds
  • Ketoglutaric Acids
  • Carbon Dioxide
  • Arginine
  • Lyases
  • ethylene forming enzyme