The proteomic profile of a mouse model of proliferative vitreoretinopathy

FEBS Open Bio. 2017 Jun 29;7(8):1166-1177. doi: 10.1002/2211-5463.12252. eCollection 2017 Aug.


Proliferative vitreoretinopathy (PVR) develops as a complication of retinal detachment surgery and represents a devastating condition leading to serious vision loss. A good animal model that permits extensive functional studies and drug testing is crucial in finding better therapeutic modalities for PVR. A previously established mouse model, using dispase injection, was analyzed from the proteomic point of view, examining global protein profile changes by 2D electrophoresis, image analysis and HPLC-tandem mass spectrometry-based protein identification. The easy applicability of the mouse model was used to study the role of transglutaminase 2 (TG2) in PVR formation by proteomic examination of dispase-induced TG2 knockout vitreous samples. Our data demonstrate that, despite the altered appearance of crystallin proteins, the lack of TG2 did not prevent the development of PVR.

Keywords: 2D electrophoresis; PVR; TG2; animal model; mass spectrometry.