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. 2018 Jan;23(1):102-108.
doi: 10.1177/2472555217722990. Epub 2017 Aug 7.

Three-Dimensional Control of Ion Channel Function Through Optogenetics and Co-Culture

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Three-Dimensional Control of Ion Channel Function Through Optogenetics and Co-Culture

Viviana Agus et al. SLAS Discov. .

Abstract

The lack of miniaturized and cost-effective methods to control cellular excitability with dosable and temporally precise electrical perturbations represents a long-lasting and unsolved bottleneck for ion channel drug discovery pipelines. Here we developed a high-throughput-compatible fluorescent-based cellular assay that combines optogenetics and co-culture approaches to obtain spatial, temporal, and quantitative control of ion channel activity. The modularity and increased flexibility of control of this light-tandem assay, combined with contained costs and compatibility with conventional drug-screening platforms, make this system suitable for temporally precise screening of ion channel function in controlled conformations and can also be used to recapitulate other complexly regulated biological processes.

Keywords: FLIPR; high-throughput screening; ion channels; optogenetics; syncytium.

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