The functional property of royal jelly 10-hydroxy-2-decenoic acid as a melanogenesis inhibitor

BMC Complement Altern Med. 2017 Aug 9;17(1):392. doi: 10.1186/s12906-017-1888-8.

Abstract

Background: It has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes. In this study, we evaluate the anti-melanogenic and depigmenting activity of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly of Apis mellifera.

Methods: In this study, we assesses the 10-HDA whitening activity in comparison with the changes in the intracellular tyrosinase activity, melanin content and melanin production related protein levles in B16F1 melanoma cells after treating with 10-HDA. Furthermore, the skin whitening effect was evaluated by applying a cream product containing with 0.5%, 1% and 2% of 10-HDA onto the skin of mice (C57BL/6 J) for 3 week to observe the effect of DL*-values.

Results: The results showed that 10-HDA inhibited the MITF protein expression (IC50 0.86 mM) in B16F1 melanoma cells. Western blot analysis revealed that 10-HDA inhibited the activity of tyrosinase and the expression of tyrosinase-related protein 1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF) in B16F1 melanoma cells. In addition, the 10-HDA was applied on the skin of mice show significantly increased the average skin-whitening index (L value).

Conclusions: The validation data indicated the potential of 10-HDA for use in suppressing skin pigmentation. The 10-HDA is proposed as a candidate to inhibit melanogenesis, thus it could be developed as cosmetics skin care products.

Keywords: 10-hydroxy-2-decenoic acid; Melanogenesis; Melanogenesis inhibitor; Royal jelly; Skin-whitening.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cosmetics
  • Down-Regulation
  • Fatty Acids / chemistry
  • Fatty Acids / pharmacology*
  • Fatty Acids, Monounsaturated / pharmacology*
  • Intramolecular Oxidoreductases / metabolism
  • Melanins / biosynthesis*
  • Mice, Inbred C57BL
  • Microphthalmia-Associated Transcription Factor / metabolism
  • Monophenol Monooxygenase / metabolism
  • Oxidoreductases / metabolism
  • Skin / drug effects*
  • Skin Pigmentation / drug effects*

Substances

  • Cosmetics
  • Fatty Acids
  • Fatty Acids, Monounsaturated
  • Melanins
  • Microphthalmia-Associated Transcription Factor
  • 10-hydroxy-2-decenoic acid
  • Oxidoreductases
  • tyrosinase-related protein-1
  • Monophenol Monooxygenase
  • Intramolecular Oxidoreductases
  • dopachrome isomerase
  • royal jelly