Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging

Chem Commun (Camb). 2017 Aug 22;53(68):9450-9453. doi: 10.1039/c7cc04966h.

Abstract

A facile method for the preparation of antibody-quantum dot (QD) conjugates using the immunoglobulin binding (B1) domain of protein G is presented. The utility of antibody-QD conjugates using the B1 domain is demonstrated for fluorescence imaging of breast tumor cells in vitro and in vivo.

MeSH terms

  • Antibodies / chemistry*
  • Binding Sites
  • Breast Neoplasms / diagnostic imaging*
  • Female
  • Humans
  • Immunoglobulins / chemistry*
  • Molecular Imaging*
  • Quantum Dots*

Substances

  • Antibodies
  • Immunoglobulins