Computation-Guided Design of a Stimulus-Responsive Multienzyme Supramolecular Assembly

Lu Yang; Elliott M Dolan; Sophia K Tan; Tianyun Lin; Eduardo D Sontag; Sagar D Khare

doi:10.1002/cbic.201700425

Computation-Guided Design of a Stimulus-Responsive Multienzyme Supramolecular Assembly

Chembiochem. 2017 Oct 18;18(20):2000-2006. doi: 10.1002/cbic.201700425. Epub 2017 Sep 1.

Authors

Lu Yang^{1

2}, Elliott M Dolan^{1

2}, Sophia K Tan², Tianyun Lin², Eduardo D Sontag^{3

2

4}, Sagar D Khare^{1

5

3

2}

Affiliations

¹ Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
² Center for Integrative Proteomics Research, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
³ Institute for Quantitative Biomedicine, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
⁴ Department of Mathematics, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
⁵ Computational Biology & Molecular Biophysics Program, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.

PMID: 28799209
DOI: 10.1002/cbic.201700425

Abstract

The construction of stimulus-responsive supramolecular complexes of metabolic pathway enzymes, inspired by natural multienzyme assemblies (metabolons), provides an attractive avenue for efficient and spatiotemporally controllable one-pot biotransformations. We have constructed a phosphorylation- and optically responsive metabolon for the biodegradation of the environmental pollutant 1,2,3-trichloropropane.

Keywords: biodegradation; enzymes; photo-crosslinking; protein design; supramolecular enzyme assembly.

MeSH terms

Computer-Aided Design*
Models, Molecular
Multienzyme Complexes / chemistry*
Propane / analogs & derivatives
Propane / chemistry
Protein Domains

Substances

Multienzyme Complexes
1,2,3-trichloropropane
Propane

Grants and funding

R01 GM100473/GM/NIGMS NIH HHS/United States