Computation-Guided Design of a Stimulus-Responsive Multienzyme Supramolecular Assembly

Chembiochem. 2017 Oct 18;18(20):2000-2006. doi: 10.1002/cbic.201700425. Epub 2017 Sep 1.


The construction of stimulus-responsive supramolecular complexes of metabolic pathway enzymes, inspired by natural multienzyme assemblies (metabolons), provides an attractive avenue for efficient and spatiotemporally controllable one-pot biotransformations. We have constructed a phosphorylation- and optically responsive metabolon for the biodegradation of the environmental pollutant 1,2,3-trichloropropane.

Keywords: biodegradation; enzymes; photo-crosslinking; protein design; supramolecular enzyme assembly.

MeSH terms

  • Computer-Aided Design*
  • Models, Molecular
  • Multienzyme Complexes / chemistry*
  • Propane / analogs & derivatives
  • Propane / chemistry
  • Protein Domains


  • Multienzyme Complexes
  • 1,2,3-trichloropropane
  • Propane