Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification

Deqin Cai; Chaochao Xun; Feng Tang; Xiaobo Tian; Liyun Yang; Kan Ding; Wenzhe Li; Zhiping Le; Wei Huang

doi:10.1016/j.carres.2017.07.011

Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification

Carbohydr Res. 2017 Sep 8:449:143-152. doi: 10.1016/j.carres.2017.07.011. Epub 2017 Aug 3.

Authors

Deqin Cai¹, Chaochao Xun², Feng Tang¹, Xiaobo Tian³, Liyun Yang³, Kan Ding³, Wenzhe Li⁴, Zhiping Le⁵, Wei Huang⁶

Affiliations

¹ CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Rd, Pudong, Shanghai, 201203, China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing, 100049, China.
² CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Rd, Pudong, Shanghai, 201203, China; Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.
³ CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Rd, Pudong, Shanghai, 201203, China.
⁴ College of Basic Medical Sciences, Dalian Medical University, 9-Western Section, Lvshun South Road, Dalian, Liaoning, 116044, China. Electronic address: liwenzhe@dlmedu.edu.cn.
⁵ Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.
⁶ CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Rd, Pudong, Shanghai, 201203, China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing, 100049, China. Electronic address: huangwei@simm.ac.cn.

PMID: 28800497
DOI: 10.1016/j.carres.2017.07.011

Abstract

Glyco-PAMAM dendrimers and glyco-agarose beads bearing a core-fucosylated N-glycan trisaccharide GlcNAcβ1,4(Fucα1,6)GlcNAc or a non-fucose disaccharide GlcNAcβ1,4GlcNAc were successfully synthesized and characterized by monosaccharide analysis with HPAEC-PAD technique. These glycoconjugates as fucose lectin probes were applied in fucose-specific lectin detection and purification. The model fucose lectin AAL indicated binding activity with the FITC-labeled PAMAM carrying core-fucose trisaccharide. An affinity chromatography column stuffed with the agarose beads carrying core-fucosylated trisaccharide exhibited a good specificity in purification of AAL than non-fucose disaccharide agarose beads. These novel glycoconjugates bearing the precise and simplified core-fucose N-glycan structure provided a potential application for core-fucose-specific lectin discovery.

Keywords: Core-fucose; Fucose-specific lectin; Glyco-agarose; Glyco-dendrimer; Glycoconjugate.

MeSH terms

Biosensing Techniques
Fucose / chemistry*
Glycoconjugates / chemistry*
Glycosylation
Lectins / chemistry*
Lectins / isolation & purification*
Microspheres
Nitrogen / chemistry
Polysaccharides / chemistry*
Trisaccharides / chemistry*

Substances

Glycoconjugates
Lectins
Polysaccharides
Trisaccharides
Fucose
Nitrogen