Dendritic Cells but Not Macrophages Sense Tumor Mitochondrial DNA for Cross-priming through Signal Regulatory Protein α Signaling

Immunity. 2017 Aug 15;47(2):363-373.e5. doi: 10.1016/j.immuni.2017.07.016. Epub 2017 Aug 8.


Inhibition of cytosolic DNA sensing represents a strategy that tumor cells use for immune evasion, but the underlying mechanisms are unclear. Here we have shown that CD47-signal regulatory protein α (SIRPα) axis dictates the fate of ingested DNA in DCs for immune evasion. Although macrophages were more potent in uptaking tumor DNA, increase of DNA sensing by blocking the interaction of SIRPα with CD47 preferentially occurred in dendritic cells (DCs) but not in macrophages. Mechanistically, CD47 blockade enabled the activation of NADPH oxidase NOX2 in DCs, which in turn inhibited phagosomal acidification and reduced the degradation of tumor mitochondrial DNA (mtDNA) in DCs. mtDNA was recognized by cyclic-GMP-AMP synthase (cGAS) in the DC cytosol, contributing to type I interferon (IFN) production and antitumor adaptive immunity. Thus, our findings have demonstrated how tumor cells inhibit innate sensing in DCs and suggested that the CD47-SIRPα axis is critical for DC-driven antitumor immunity.

MeSH terms

  • Animals
  • Antibodies, Blocking / therapeutic use
  • Antigens, Differentiation / metabolism*
  • CD47 Antigen / immunology
  • CD47 Antigen / metabolism
  • Cells, Cultured
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / immunology*
  • Colonic Neoplasms / therapy
  • Cross-Priming
  • DNA, Mitochondrial / immunology*
  • Dendritic Cells / immunology*
  • Disease Models, Animal
  • Humans
  • Interferon Type I / metabolism
  • Macrophages / immunology
  • Membrane Glycoproteins / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NADPH Oxidase 2
  • NADPH Oxidases / metabolism
  • Nucleotidyltransferases / metabolism
  • Receptors, Immunologic / metabolism*
  • Signal Transduction
  • Tumor Escape


  • Antibodies, Blocking
  • Antigens, Differentiation
  • CD47 Antigen
  • DNA, Mitochondrial
  • Interferon Type I
  • Membrane Glycoproteins
  • Membrane Proteins
  • Ptpns1 protein, mouse
  • Receptors, Immunologic
  • SIRPA protein, human
  • Sting1 protein, mouse
  • CYBB protein, human
  • NADPH Oxidase 2
  • NADPH Oxidases
  • Nucleotidyltransferases
  • cGAS protein, mouse