How do red and infrared low-level lasers affect folliculogenesis cycle in rat's ovary tissue in comparison with clomiphene under in vivo condition

Lasers Med Sci. 2017 Dec;32(9):1971-1979. doi: 10.1007/s10103-017-2296-5. Epub 2017 Aug 11.


Folliculogenesis is a cycle that produces the majority of oocyte. Any disruption to this cycle leads to ovulation diseases, like polycystic ovarian syndrome (PCOS). Treatments include drugs and surgery; lasers have also been used complementarily. Meanwhile, still there is no definite treatment for PCOS. This study investigated the photo-bio stimulation effect of near-infrared and red low-level laser on producing follicles and compared the result with result of using common drug, clomiphene. Therefore, the aim of this study was to propose the use of lasers autonomously treatment. So, there was one question: how do lasers affect folliculogenesis cycle in rat's ovary tissue? In this study, 28 rats were assigned to four groups as follows: control (CT), clomiphene drug (D), red laser (RL), and near-infrared laser (NIRL). Afterwards, 14 rats of RL and NIRL groups received laser on the first 2 days of estrous cycle, each 6 days, for 48 days. During treatment period, each rat received energy density of 5 J/cm2. Seven rats in D group received clomiphene. After the experiment, lasers' effects at two wavelengths of 630 and 810 nm groups have been investigated and compared with clomiphene and CT groups. Producing different follicles to complement folliculogenesis cycle increased in NIRL and RL groups, but this increase was significant only in the NIRL group. This indicates that NIRL increases ovarian activity to produce oocyte that certainly can be used in future studies for finding a cure to ovarian negligence to produce more oocyte and treat diseases caused by it like PCOS.

Keywords: Clomiphene drug; Folliculogenesis cycle; Near-infrared laser; Polycystic ovarian syndrome; Rat ovary tissue; Red laser.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Clomiphene / pharmacology*
  • Female
  • Fertility Agents, Female / pharmacology
  • Hormones / pharmacology
  • Infrared Rays*
  • Low-Level Light Therapy*
  • Ovarian Follicle / drug effects*
  • Ovarian Follicle / pathology
  • Ovarian Follicle / physiology
  • Ovarian Follicle / radiation effects*
  • Rats, Wistar


  • Fertility Agents, Female
  • Hormones
  • Clomiphene