Abstract
GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated successful protocol for processing paraffin-embedded tissue and assess the feasibility of the Xpert assay-based tuberculosis (TB) diagnosis on these specimens, thus proving the Xpert assay as a valuable TB diagnostic tool in supporting conventional histopathological methods.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibiotics, Antitubercular / pharmacology
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Automation, Laboratory
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Bacteriological Techniques*
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Biopsy
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Case-Control Studies
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DNA, Bacterial / genetics*
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DNA, Bacterial / isolation & purification
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Drug Resistance, Bacterial / genetics
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Humans
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Microbial Sensitivity Tests
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Mycobacterium tuberculosis / drug effects
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Mycobacterium tuberculosis / genetics*
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Mycobacterium tuberculosis / isolation & purification
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Paraffin Embedding*
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Predictive Value of Tests
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Real-Time Polymerase Chain Reaction / methods*
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Rifampin / pharmacology
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Tuberculosis, Lymph Node / diagnosis*
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Tuberculosis, Lymph Node / genetics
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Tuberculosis, Lymph Node / pathology
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Tuberculosis, Pleural / diagnosis*
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Tuberculosis, Pleural / genetics
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Tuberculosis, Pleural / pathology
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Tuberculosis, Pulmonary / diagnosis*
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Tuberculosis, Pulmonary / genetics
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Tuberculosis, Pulmonary / pathology
Substances
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Antibiotics, Antitubercular
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DNA, Bacterial
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Rifampin