Performance of Activated Partial Thromboplastin Time (APTT): Determining Reagent Sensitivity to Factor Deficiencies, Heparin, and Lupus Anticoagulants

Geoffrey Kershaw

doi:10.1007/978-1-4939-7196-1_5

Performance of Activated Partial Thromboplastin Time (APTT): Determining Reagent Sensitivity to Factor Deficiencies, Heparin, and Lupus Anticoagulants

Methods Mol Biol. 2017:1646:75-83. doi: 10.1007/978-1-4939-7196-1_5.

Author

Geoffrey Kershaw¹

Affiliation

¹ Institute of Haematology, Royal Prince Alfred Hospital, Missenden Road, Camperdown, NSW, 2050, Australia. geoffrey.kershaw@sswahs.nsw.gov.au.

PMID: 28804819
DOI: 10.1007/978-1-4939-7196-1_5

Abstract

The activated partial thromboplastin time (APTT) is a useful global assay for the assessment of the contact factor pathway of hemostasis and its inhibitors. The test is usually performed on fully automated analyzers using commercially prepared reagents. The three main clinical areas of interest are detection of factor deficiencies, detection of lupus anticoagulants and in the monitoring of therapy with unfractionated heparin. Methods are described here for assessing APTT reagents for their sensitivity to clotting time prolongation in each of these areas of interest.

Keywords: Factor deficiency; Heparin; Lupus anticoagulants.

MeSH terms

Anticoagulants / therapeutic use*
Blood Coagulation* / drug effects
Coagulation Protein Disorders / blood*
Coagulation Protein Disorders / diagnosis
Drug Monitoring / methods*
Heparin / therapeutic use*
Humans
Indicators and Reagents
Lupus Coagulation Inhibitor / blood*
Partial Thromboplastin Time / methods*

Substances

Anticoagulants
Indicators and Reagents
Lupus Coagulation Inhibitor
Heparin