Automated synthesis of 1-[11C]acetoacetate on a TRASIS AIO module

Appl Radiat Isot. 2017 Nov:129:57-61. doi: 10.1016/j.apradiso.2017.07.066. Epub 2017 Aug 2.


We automated radiochemical synthesis of 1-[11C]acetoacetate in a commercially available radiochemistry module, TRASIS AllInOne by [11C]carboxylation of the corresponding enolate anion generated in situ from isopropenylacetate and MeLi, and purified by ion-exchange column resins.1-[11C]acetoacetate was synthesized with high radiochemical purity (95%) and specific activity (~ 66.6GBq/µmol, n = 30) with 35% radiochemical yield, decay corrected to end of synthesis. The total synthesis required ~ 16min. PET imaging studies were conducted with 1-[11C]acetoacetate in vervet monkeys to validate the radiochemical synthesis. Tissue uptake distribution was similar to that reported in humans.

Keywords: Automation; Formulations; Ion-exchange column; Ketone metabolism; Non-Human primates; PET imaging.