Due to our intensive subsistence and habitat-modification strategies-including broad-spectrum harvesting and predation, widespread landscape burning, settlement construction, and translocation of other species-humans have major roles as ecological actors who influence fundamental trophic interactions. Here we review how the long-term history of human-environment interaction has shaped the evolutionary biology of diverse non-human, non-domesticated species. Clear examples of anthropogenic effects on non-human morphological evolution have been documented in modern studies of substantial changes to body size or other major traits in terrestrial and aquatic vertebrates, invertebrates, and plants in response to selective human harvesting, urbanized habitats, and human-mediated translocation. Meanwhile, archaeological records of harvested marine invertebrates and terrestrial vertebrates suggest that similar processes extend considerably into prehistory, perhaps to 50,000 yr BP or earlier. These results are consistent with palaeoenvironmental and other records that demonstrate long-term human habitat modification and intensive harvesting practices. Thus, while considerable attention has been focused on recent human impacts on 'natural' habitats, integrated evidence from modern biology and archaeology suggests a deep history of human entanglement with our ecosystems including substantial effects on the evolutionary biology of non-human taxa. The number and magnitude of such effects will probably increase given the continued intensification of anthropogenic activities and ecosystem impacts, including climate change and direct genetic modification.