Genomic characterization of a novel calicivirus, FHMCV-2012, from baitfish in the USA

Arch Virol. 2017 Dec;162(12):3619-3627. doi: 10.1007/s00705-017-3519-6. Epub 2017 Aug 16.


During regulatory sampling of fathead minnows (Pimephales promelas), a novel calicivirus was isolated from homogenates of kidney and spleen inoculated into bluegill fry (BF-2) cells. Infected cell cultures exhibiting cytopathic effects were screened by PCR-based methods for selected fish viral pathogens. Illumina HiSeq next generation sequencing of the total RNA revealed a novel calicivirus genome that showed limited protein sequence similarity to known homologs in a BLASTp search. The complete genome of this fathead minnow calicivirus (FHMCV) is 6564 nt long, encoding a polyprotein of 2114 aa in length. The complete polyprotein shared only 21% identity with Atlantic salmon calicivirus,followed by 11% to 14% identity with mammalian caliciviruses. A molecular detection assay (RT-PCR) was designed from this sequence for screening of field samples for FHMCV in the future. This virus likely represents a prototype species of a novel genus in the family Caliciviridae, tentatively named "Minovirus".

MeSH terms

  • Animal Structures / virology
  • Animals
  • Caliciviridae / classification*
  • Caliciviridae / genetics
  • Caliciviridae / isolation & purification*
  • Caliciviridae Infections / veterinary*
  • Caliciviridae Infections / virology
  • Cells, Cultured
  • Cyprinidae / virology*
  • Cytopathogenic Effect, Viral
  • Genome, Viral*
  • Genomics
  • Kidney / virology
  • Phylogeny*
  • Polymerase Chain Reaction
  • RNA, Viral / genetics
  • Sequence Analysis, DNA
  • Sequence Homology
  • Spleen / virology
  • United States
  • Viral Proteins / genetics
  • Virus Cultivation


  • RNA, Viral
  • Viral Proteins