Cancer cytokines are known to mediate several complex cancer cell physiologies. Also, cancer cells themselves are known to secrete cytokines whose expressions and net inducible results are controlled by a variety of factors. We profiled a few cytokines secreted by 2D, 3D aggregates, and the 3DRs of MCF-7 cell line at various time points. Several cytokines were seen more expressed by 3D cultures on the 4th day and IL-10 peaked on the day 1 of 3D cultures while TNF-α level peaked on the 7th day. α-Defensin, SDF-7, and TGF-β also showed markedly higher levels. There was a reduced expression of IL-6 and IL-17 by the 3DRs. TGF-β did not show much change among the 2D, progressive 3D, and 3DR cultures. Utilizing 3DRs as study material will be a significant extension of the ways cells lines can be used for research.
Keywords: 3D aggregates; 3D reverts; MCF-7; cytokines.
© 2017 Wiley Periodicals, Inc.