Potential effect of compounds isolated from Coffea arabica against UV-B induced skin damage by protecting fibroblast cells

J Photochem Photobiol B. 2017 Sep:174:323-332. doi: 10.1016/j.jphotobiol.2017.08.015. Epub 2017 Aug 9.


Ultraviolet (UV) radiation has adverse effects on extracellular matrix (ECM) proteins, leading to formation of wrinkles a hallmark of premature skin aging. The adverse effects of UV radiation are associated with induction of matrix metalloproteinases (MMPs) expression and degradation of collagen and elastin. The present study investigated anti-wrinkle effects of chlorogenic acid (CGA), pyrocatechol (PC) and 3,4,5-tricaffeoyl quinic acid (TCQ), isolated from beans of Coffea arabica, against UV-B stimulated mouse fibroblast cells (CCRF) by measuring expression levels of MMP-1, 3, 9, and type-I procollagen. The three compounds were isolated and purified from coffee grounds using column chromatography and structural examination was evaluated by nuclear magnetic resonance (NMR) analysis. Among the three isolated compounds, CGA effectively suppressed the expression of the MMP-1, 3, and 9 and increased synthesis of type-I procollagen as compared UV-B-stimulated CCRF cells. In addition, CGA dose-dependently inhibited intracellular reactive oxygen species (ROS) production in CCRF cells stimulated by UV radiation. Moreover, CGA displayed a good sun protection factor (SPF) and in vitro DNA damage protection together with inhibition of enzyme xanthine oxidase. The enzyme inhibitory kinetic behavior of CGA was determined by Lineweaver-Burk plot, displayed a mixed type enzyme inhibition with 260.3±4.5μM, Ki value. The results indicate that CGA has potential to be used as a preventive agent against premature skin aging induced by UV radiation.

Keywords: 3,4,5-Tricaffeoyl quinic; Chlorogenic acid; MMP-1, 3, 9; Pyrocatechol; Sun protection factor; Type-I procollagen.

MeSH terms

  • Animals
  • Cell Line
  • Coffea / chemistry*
  • Collagen Type I / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Fibroblasts / radiation effects*
  • Gene Expression Regulation, Enzymologic / drug effects
  • Intracellular Space / drug effects
  • Intracellular Space / metabolism
  • Intracellular Space / radiation effects
  • Matrix Metalloproteinases / metabolism
  • Mice
  • Plant Extracts / isolation & purification*
  • Plant Extracts / pharmacology*
  • Radiation-Protective Agents / isolation & purification
  • Radiation-Protective Agents / pharmacology
  • Reactive Oxygen Species / metabolism
  • Ultraviolet Rays / adverse effects*
  • Xanthine Oxidase / metabolism


  • Collagen Type I
  • Plant Extracts
  • Radiation-Protective Agents
  • Reactive Oxygen Species
  • Xanthine Oxidase
  • Matrix Metalloproteinases