Draft Genome Sequences of Escherichia coli O104 Strains of Bovine and Human Origin

Pragathi B Shridhar; Isha R Patel; Jayanthi Gangiredla; Mark K Mammel; Lance Noll; Xiaorong Shi; Jianfa Bai; Christopher A Elkins; Nancy Strockbine; T G Nagaraja

doi:10.1128/genomeA.00630-17

Draft Genome Sequences of Escherichia coli O104 Strains of Bovine and Human Origin

Genome Announc. 2017 Aug 17;5(33):e00630-17. doi: 10.1128/genomeA.00630-17.

Authors

Affiliations

¹ Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
² Center for Food Safety and Applied Nutrition, Division of Molecular Biology, United States Food and Drug Administration, Laurel, Maryland, USA.
³ Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas, USA.
⁴ National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Division of Foodborne, Waterborne and Environmental Diseases, Atlanta, Georgia, USA.
⁵ Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA tnagaraj@vet.k-state.edu.

Abstract

Cattle harbor and shed in their feces several Escherichia coli O104 serotypes. All O104 strains examined were intimin negative and belonged to the B1 phylogroup, and some were Shiga toxigenic. We report here the genome sequences of bovine O104:H7 (n = 5), O104:H23 (n = 2), O104:H8 (n = 1), and O104:H12 (n = 1) isolates and human clinical isolates of O104:H7 (n = 5).