Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells

Sci Rep. 2017 Aug 17;7(1):8519. doi: 10.1038/s41598-017-04878-0.

Abstract

Airway epithelial cell-derived thymic stromal lymphopoietin (TSLP) and IL-33 can enhance lung-resident group 2 innate lymphoid cells (ILC2s), and they play an important role in the development of allergic diseases. This study tests the hypothesis that Der f 31 (Dermatophagoides farinae-31), an allergen, modulates airway epithelial cell functions and increases the frequency of lung ILC2s. Our previous research identified cofilin (Der f 31) as a novel allergen. In this study, we found that recombinant Der f 31 (r-Der f 31) upregulated the expression of co-stimulatory molecules in DCs and promoted Th2-skewed polarization. The levels of TSLP and IL-33 in epithelial cells were upregulated by r-Der f 31 via the activation of Toll-like receptor 2. Furthermore, in in vivo studies, r-Der f 31 induced eosinophil-like airway allergy and increased the number of lung-resident ILC2s. In summary, Der f 31 can modulate the functions of airway epithelial cells and increase levels of lung-resident ILC2s.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Depolymerizing Factors / immunology
  • Allergens / immunology*
  • Animals
  • Antigens, Dermatophagoides / immunology*
  • Cells, Cultured
  • Cytokines / metabolism*
  • Dermatophagoides farinae / immunology
  • Epithelial Cells / drug effects*
  • Epithelial Cells / metabolism*
  • Humans
  • Lung / pathology*
  • Lymphocytes / immunology*
  • Mice, Inbred BALB C
  • Models, Biological
  • Thymic Stromal Lymphopoietin

Substances

  • Actin Depolymerizing Factors
  • Allergens
  • Antigens, Dermatophagoides
  • Cytokines
  • Thymic Stromal Lymphopoietin