A selective glucose sensor based on direct oxidation on a bimetal catalyst with a molecular imprinted polymer

Biosens Bioelectron. 2018 Jan 15:99:471-478. doi: 10.1016/j.bios.2017.08.022. Epub 2017 Aug 10.


A selective nonenzymatic glucose sensor was developed based on the direct oxidation of glucose on hierarchical CuCo bimetal-coated with a glucose-imprinted polymer (GIP). Glucose was introduced into the GIP composed of Nafion and polyurethane along with aminophenyl boronic acid (APBA), which was formed on the bimetal electrode formed on a screen-printed electrode. The extraction of glucose from the GIP allowed for the selective permeation of glucose into the bimetal electrode surface for oxidation. The GIP-coated bimetal sensor probe was characterized using electrochemical and surface analytical methods. The GIP layer coated on the NaOH pre-treated bimetal electrode exhibited a dynamic range between 1.0µM and 25.0mM with a detection limit of 0.65±0.10µM in phosphate buffer solution (pH 7.4). The anodic responses of uric acid, acetaminophen, dopamine, ascorbic acid, L-cysteine, and other saccharides (monosaccharides: galactose, mannose, fructose, and xylose; disaccharides: sucrose, lactose, and maltose) were not detected using the GIP-coated bimetal sensor. The reliability of the sensor was evaluated by the determination of glucose in artificial and whole blood samples.

Keywords: Aminophenyl boronic acid; Electrochemical glucose detection; Glucose-imprinted polymer; Hierarchical CuCo catalyst; Nonenzymatic sensor.

MeSH terms

  • Biosensing Techniques*
  • Blood Glucose / chemistry
  • Blood Glucose / isolation & purification*
  • Boronic Acids / chemistry
  • Catalysis
  • Glucose / chemistry
  • Glucose / isolation & purification*
  • Limit of Detection
  • Molecular Imprinting*
  • Nanotubes, Carbon / chemistry
  • Oxidation-Reduction
  • Polymers / chemistry


  • Blood Glucose
  • Boronic Acids
  • Nanotubes, Carbon
  • Polymers
  • 3-aminobenzeneboronic acid
  • Glucose