The Cell-Cycle Regulated Proliferating Cell Nuclear Antigen Is Required for SV40 DNA Replication in Vitro

Nature. 1987 Apr 2-8;326(6112):471-5. doi: 10.1038/326471a0.


Cell-free extracts prepared from human 293 cells, supplemented with purified SV40 large-T antigen, support replication of plasmids containing the SV40 origin of DNA replication. A cellular protein (Mr approximately 36,000) that is required for efficient SV40 DNA synthesis in vitro has been purified from these extracts. This protein is recognized by human autoantibodies and is identified as the cell-cycle regulated protein known as proliferating cell nuclear antigen (PCNA) or cyclin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Autoantigens*
  • Cell Cycle
  • Cell Line
  • Cell-Free System
  • DNA Replication*
  • Humans
  • Kinetics
  • Nucleoproteins / physiology*
  • Plasmids
  • Proliferating Cell Nuclear Antigen
  • Simian virus 40 / genetics*


  • Autoantigens
  • Nucleoproteins
  • Proliferating Cell Nuclear Antigen