Planting of neonicotinoid-coated corn raises honey bee mortality and sets back colony development

PeerJ. 2017 Aug 14;5:e3670. doi: 10.7717/peerj.3670. eCollection 2017.

Abstract

Worldwide occurrences of honey bee colony losses have raised concerns about bee health and the sustainability of pollination-dependent crops. While multiple causal factors have been identified, seed coating with insecticides of the neonicotinoid family has been the focus of much discussion and research. Nonetheless, few studies have investigated the impacts of these insecticides under field conditions or in commercial beekeeping operations. Given that corn-seed coating constitutes the largest single use of neonicotinoid, our study compared honey bee mortality from commercial apiaries located in two different agricultural settings, i.e. corn-dominated areas and corn-free environments, during the corn planting season. Data was collected in 2012 and 2013 from 26 bee yards. Dead honey bees from five hives in each apiary were counted and collected, and samples were analyzed using a multi-residue LC-MS/MS method. Long-term effects on colony development were simulated based on a honey bee population dynamic model. Mortality survey showed that colonies located in a corn-dominated area had daily mortality counts 3.51 times those of colonies from corn crop-free sites. Chemical analyses revealed that honey bees were exposed to various agricultural pesticides during the corn planting season, but were primarily subjected to neonicotinoid compounds (54% of analysed samples contained clothianidin, and 31% contained both clothianidin and thiamethoxam). Performance development simulations performed on hive populations' show that increased mortality during the corn planting season sets back colony development and bears contributions to collapse risk but, most of all, reduces the effectiveness and value of colonies for pollination services. Our results also have implications for the numerous large-scale and worldwide-cultivated crops that currently rely on pre-emptive use of neonicotinoid seed treatments.

Keywords: Apis mellifera; Clothianidin; Colony health; Honey bee; Insecticide; Intoxication; Pollinator; Population dynamic model; Seed treatment; Thiamethoxam.

Grant support

This study was funded by Prime-Vert sous volet 11,1—Appui à la Stratégie phytosanitaire québécoise en agriculture of the Ministère de l’Agriculture, des Pêcherie et de l’Alimentation (MAPAQ). OS-R received various scholarships from the Quebec’s Centre for Biodiversity Science and NSERC-CANPOLIN. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.