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. 2018 Mar;57(3):487-493.
doi: 10.1002/mus.25775. Epub 2017 Aug 31.

Messenger RNA Profiling of Rabbit Quadriceps Femoris After Repeat Injections of Botulinum Toxin: Evidence for a Dynamic Pattern Without Further Structural Alterations

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Messenger RNA Profiling of Rabbit Quadriceps Femoris After Repeat Injections of Botulinum Toxin: Evidence for a Dynamic Pattern Without Further Structural Alterations

David A Hart et al. Muscle Nerve. .

Abstract

Introduction: Onabotulinum toxin type A (BoNT-A) is widely used clinically, but it may cause adverse effects. Earlier studies showed repeat BoNT-A injections did not cause progressive atrophy or function loss. The purpose of this study was to assess the influence of repeat BoNT-A injections into rabbit muscle on subsequent molecular alterations.

Methods: Twenty-two rabbits received 0, 1, 2, or 3 BoNT-A injections in the quadriceps femoris muscle at 3-month intervals and were euthanized 6 months after the last injection. Aliquots of both injected and contralateral muscle were frozen, and the total RNA quantified.

Results: Total RNA per illigram wet weight tissue was significantly elevated compared with control after 1, 2, or 3 BoNT-A injections. Analysis of mRNA levels for inflammatory molecules, proteinases, adipokines, and mesenchymal stem cells were elevated with increasing BoNT-A injections in injected leg and contralateral leg.

Discussion: Future studies should focus on the safety and possible complications of repeat BoNT-A treatments. Muscle Nerve 57: 487-493, 2018.

Keywords: NZW rabbits; botulinum toxin-A; mRNA assessments; mesenchymal stem cells; muscle atrophy; muscle weakness.

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