The in vitro antibacterial activity of incomplete iron salt of polyacrylic acid against Pseudomonas aeruginosa, meticillin-resistant Staphylococcus pseudintermedius and meticillin-resistant S. aureus

Vet Dermatol. 2018 Feb;29(1):3-e2. doi: 10.1111/vde.12483. Epub 2017 Aug 22.

Abstract

Background: The incomplete iron salt of polyacrylic acid (IIS-PAA) has been used for its haemostatic, wound healing and antibacterial properties in human patients. IIS-PAA is a water-soluble, acidic, hydrophilic polymer that is active at pH 2.9-4.0.

Hypotheses/objectives: To evaluate the antibacterial effect of IIS-PAA against Pseudomonas aeruginosa, meticillin-resistant Staphylococcus pseudintermedius (MRSP) and meticillin-resistant S. aureus (MRSA).

Methods: This was a prospective, nonblinded in vitro study. Sixty canine isolates of P. aeruginosa, MRSP and MRSA were included. Minimum inhibitory concentrations (MICs) of IIS-PAA were determined by agar dilution on Mueller-Hinton agar using testing concentrations ranging from 0.0625-0.4% (raw material 100%) and based on Clinical and Laboratory Standards Institute guidelines.

Results: The median MIC for P. aeruginosa was 0.2% (range 0.2-0.3%), mean 0.2% and standard deviation (SD) of 0.018%. The median MIC for MRSP was 0.05% (0.025-0.1%), mean 0.058% and SD 0.022%, and the median MIC for MRSA was 0.1% (0.00625-0.4%), mean 0.126% and SD 0.080%. There were significant differences in MICs between the three bacteria types with MICs highest for Pseudomonas and lowest for MRSP (all P < 0.0001).

Conclusions: IIS-PAA had in vitro antibacterial activity against three important bacterial pathogens. Further studies of IIS-PAA are indicated to assess the potential clinical benefit and in vitro studies to investigate the spectrum of anti-bacterial activity and mode of action.

MeSH terms

  • Acrylic Resins / chemistry
  • Acrylic Resins / pharmacology*
  • Animals
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Dogs
  • Ferric Compounds / chemistry
  • Ferric Compounds / pharmacology*
  • In Vitro Techniques
  • Iron / pharmacology*
  • Methicillin Resistance
  • Methicillin-Resistant Staphylococcus aureus / drug effects*
  • Microbial Sensitivity Tests / veterinary
  • Pseudomonas aeruginosa / drug effects*
  • Staphylococcus / drug effects*

Substances

  • Acrylic Resins
  • Anti-Bacterial Agents
  • Ferric Compounds
  • IIS-PAA
  • carbopol 940
  • Iron